Indophenol Blue Method for Ammonia Analysis
Indophenol Blue Method for Ammonia Analysis
Embedded Files
Reagent Preparation for Indophenol-Blue
(Alkaline Samples)
Reagent Preparation for Indophenol-Blue
(Alkaline Samples)
Reagent A:
1 M NaOH
5 wt% Salicylic Acid
5 wt% Trisodium Citrate Dihydrate
Reagent B:
0.05 M NaClO
Reagent C:
1 wt% Sodium Pentacyanonitrosylferrate
Indophenol-Blue Procedure
(Alkaline Samples)
Indophenol-Blue Procedure
(Alkaline Samples)
Sample Solution: 1.33 mL
Reagent A: 1.33 mL
Reagent B: 0.64 mL
Reagent C: 0.13 mL
→ Wait for 1 h ←
(all mixed in plastic cuvette)
Acorrected = A655 - A800
Calibration curve updated (05-25-26)
Reagent Preparation for Indophenol-Blue
(Neutral Buffered & Acidic Samples)
Reagent Preparation for Indophenol-Blue
(Neutral Buffered & Acidic Samples)
Reagent A:
1 M NaOH
5 wt% Salicylic Acid
5 wt% Trisodium Citrate Dihydrate
Reagent B:
0.05 M NaClO
Reagent C:
1 wt% Sodium Pentacyanonitrosylferrate
Reagent D:
2 M NaOH
Indophenol-Blue Procedure
(Neutral Buffered & Acidic Samples)
Indophenol-Blue Procedure
(Neutral Buffered & Acidic Samples)
Sample Solution: 1.064 mL
Reagent A: 1.33 mL
Reagent B: 0.64 mL
Reagent C: 0.13 mL
Reagent C: 0.266 mL
→ Wait for 1 h ←
(all mixed in plastic cuvette)
Acorrected = A655 - A800
Calibration curve updated (05-25-26)
Want to learn more about the chemistry behind this analysis?
Griess Test for Nitrite Analysis
Griess Test for Nitrite Analysis
Reagent Preparation for
Griess Reagent
Reagent Preparation for
Griess Reagent
Reagent A (Griess Reagent):
0.387 M Sulfanilamide
0.013 M N-(1-naphthyl)ethylenediamine dihydrochloride (NED)
2.45 M H3PO4
Reagent B:
H3PO4 (85%)
Griess Test Procedure
Griess Test Procedure
Sample Solution: 3.36 mL
Reagent A: 70 µL
Reagent B: 70 µL
→ Wait for 20 min ←
(all mixed in plastic cuvette)
Acorrected = A540 - A800
Calibration curve updated (05-25-26)
Want to learn more about the chemistry behind this analysis?
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